The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome.
- Author:
Li-qun FANG
1
;
Pan-he ZHANG
;
Bao-an YANG
;
Xiao-ming WU
;
Qiu-min ZHAO
;
Wei LIU
;
Hong LIU
;
Yong-qiang DENG
;
Lin ZHAN
;
Wei-guo HAN
;
Fu-shuang LU
;
Jin-song WU
;
Hong YANG
;
Qing-yu ZHU
;
Wu-chun CAO
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Viral; blood; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique, Indirect; methods; Humans; Immunoglobulin G; blood; Immunoglobulin M; blood; SARS Virus; immunology; Severe Acute Respiratory Syndrome; diagnosis
- From:Chinese Journal of Epidemiology 2003;24(6):484-486
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS.
METHODSClinically confirmed SARS patients, suspected SARS patients, and controls were included in the study. IFA was used to detect the serum antibody against SARS coronavirus. General information about the subjects was collected using a standard questionnaire.
RESULTSThe positive rates of specific IgG and IgM against SARS virus within 10 days after onset of the disease were 55.1% and 16.3% respectively and then increased up to 89.8% for IgG and 65.3% for IgM. After 25 days of the onset of the disease, 90.9% patients became positive for both IgG and IgM. Results from chi-square for trend test revealed that the positive rates of both IgG and IgM increased with time (chi(2) for trend = 16.376, P = 0.00005 for IgG; chi(2) for trend = 28.736, P = 0.00000 for IgM). Sensitivity, specificity and agreement value of IFA regarding the diagnosis of SARS were all higher than 90%.
CONCLUSIONIFA can be used to assist diagnosis of SARS after 10 days of the onset of disease.
