Rapid detection of genotypes of TT virus using a heteroduplex mobility assay.
- Author:
Zhong-ping HE
1
;
Hui ZHUANG
;
Jun YAO
;
Qing-ming DONG
;
Wang-su DAI
;
Shu-jing SONG
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Viral; analysis; Genotype; Hepatitis, Viral, Human; virology; Heteroduplex Analysis; methods; Humans; Phylogeny; Torque teno virus; classification; genetics
- From:Chinese Journal of Epidemiology 2003;24(9):801-805
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a simple, sensitive, specific and less-costly method for detecting genotypes of TT virus (TTV).
METHODSTTV DNA was tested by nested polymerase chain reaction (nPCR) in sera from 180 patients with different types of viral hepatitis and 96 normal individuals in Beijing. TTV genotypes were determined in 40 sera collected from TTV DNA positive patients by heteroduplex mobility assay (HMA) and through sequencing.
RESULTSThe positive rates of TTV DNA in viral hepatitis patients and normal individuals were 22.2% (40/180) and 19.8% (19/96), respectively (chi(2) = 0.220, P = 0.639). TTV DNA positive rates of patients with hepatitis A, B, C, E and non-A to E were 20.0% (6/30), 16.7% (5/30), 23.3% (7/30), 36.7% (11/30) and 18.3% (11/60), respectively. Of 40 TTV DNA positive patients, 20 (50.0%) were TTV G1, 7 (17.5%) TTV G2, 10 (25.0%) coinfected with different genotypes of TTV, and 3 untyped by HMA. Twenty G1 and 7 G2 detected by HMA were confirmed by sequence analysis. Of 10 patients coinfected with different genotypes of TTV, 5 were G1 and G2, 2 G1 and G3, 1 G1 and G4, 1 G1 and G3, and 1 with G1, G2 and G3 coinfections.
CONCLUSIONHMA was recognized as simple, sensitive, specific and less-costly, thus could be used for genotyping of TTV.
