Relationship between DLC-1 expressions and metastasis in hepatocellular carcinoma.
- Author:
Li-jie SONG
1
;
Sheng-long YE
;
Kai-feng WANG
;
Yong-qiang WENG
;
Chun-min LIANG
;
Rui-xia SUN
;
Yan ZHAO
;
Yin-kun LIU
;
Zhao-you TANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; metabolism; pathology; Cell Line, Tumor; GTPase-Activating Proteins; Gene Expression Regulation, Neoplastic; Humans; Liver Neoplasms; metabolism; pathology; Mutagenesis, Site-Directed; Neoplasm Metastasis; Neoplasm Recurrence, Local; RNA, Messenger; biosynthesis; genetics; Tumor Suppressor Proteins; biosynthesis; genetics
- From: Chinese Journal of Hepatology 2005;13(6):428-431
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo study the relationship between the expression level of DLC-1 mRNA (located in 8p) and the invasion/metastasis of human hepatocellular carcinoma (HCC).
METHODSFifty-one surgical specimens of human HCC were divided into high-invasive and low invasive groups according to their clinicopathological features. DLC-1 mRNA expression was studied in the 51 HCC specimens as well as 5 different metastasis potential cell lines using real-time quantitative PCR (RQ-PCR).
RESULTSThe expression level of DLC-1 mRNA in HCC specimens with high invasiveness was significantly lower than that with low invasiveness (P < 0.05). The expression levels of DLC-1 mRNA were significantly different between non-metastatic (Hep3B and HepG2) and metastatic (MHCC97-H, MHCC97-L and HCCLM3) cell lines (P < 0.05). From MHCC97-L to HCCLM3, with an increase of invasiveness and metastatic potentials, the expression level of DLC-1 decreased correspondingly, and its expression level in HCCLM3 was significantly lower than that in MHCC97-L (P < 0.01).
CONCLUSIONThe expression of DLC-1 mRNA may play an important role in inhibiting the invasiveness and metastasis of HCC.
