Differential analysis of tyrosine-phosphorylated proteins in human hepatocellular carcinoma cell lines with different metastasis potentials.
- Author:
Li-jun ZHANG
1
;
Jie-feng CUI
;
Yin-kun LIU
;
Yun-song ZHU
;
Hai-yan SONG
;
Hua-li SHEN
;
Ju-tao FENG
;
Zhi DAI
;
Jie CHEN
;
Rui-xia SUN
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; metabolism; pathology; Cell Line, Tumor; Electrophoresis, Gel, Two-Dimensional; Humans; Liver Neoplasms; metabolism; pathology; Neoplasm Metastasis; Neoplasm Proteins; analysis; Phosphotyrosine; analysis
- From: Chinese Journal of Hepatology 2005;13(6):436-439
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo compare expressions of tyrosine-phosphorylated proteins in different hepatocellular carcinoma cell lines with different metastasis potential and to screen key molecules associated with HCC metastasis and recurrence.
METHODSUsing two-dimensional electrophoresis, Western blotting and MALDI-TOF-MS/MS, we analyzed tyrosine-phosphorylated protein profiles of Hep3B, MHCC97L and MHCC97H, HCC cell lines with different metastasis potentials.
RESULTS10 spots were detected in Hep3B, 19 in MHCC97L and 17 in MHCC97H. Seventeen significantly different phosphotyrosine proteins in gel were identified by MALDI-TOF-MS/MS, including Annexin I.
CONCLUSIONThe changed expression of tyrosine-phosphorylated proteins is associated with HCC metastasis and recurrence.
