Effects of gamma-interferon on gene expression of collagen I, III and on the tissue inhibitor of metalloprotenase 1 in HSC-T6 cells.
- Author:
Hong MA
1
;
Xue-mei MA
;
Cheng-hong YIN
;
Ji-dong JIA
;
Bao-en WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Collagen Type I; biosynthesis; genetics; Collagen Type III; biosynthesis; genetics; Hepatocytes; cytology; metabolism; Interferon-gamma; pharmacology; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Sprague-Dawley; Tissue Inhibitor of Metalloproteinase-1; biosynthesis; genetics
- From: Chinese Journal of Hepatology 2005;13(7):528-530
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the in vitro effects of gamma-interferon (IFNgamma) on gene expression of collagen I (Col I), III (Col III) and tissue inhibitor of metalloprotenase 1 (TIMP1) of HSC-T6 cells.
METHODSCultured HSC-T6 cells were exposed to IFNgamma at concentrations of 0.1, 1, 10, 10(2), 10(3), 10(4), 2.5 x 10(5), 5 x10(5) U/ml for 48 hours. 4,5-simethylthiazaoly colormetric assay was used to evaluate the effect of IFNgamma on HSC-T6 cell proliferation. After incubating with IFNgamma (1 U/ml, 10(2) U/ml and 10(4) U/ml) for 48 hours, HSC-T6 cells were harvested to detect Col I, Col III and TIMP1 steady state mRNA levels by quantitative reverse-transcription polymerase chain reaction (RT-PCR).
RESULTSThe Col I, Col III and TIMP1 mRNA levels of the control group were 2.86+/-0.21, 2.00+/-0.23 and 3.90+/-0.81, respectively. Col I and Col III mRNA levels in HSC-T6 cells treated by different concentrations of IFNgamma were lower than that of the controls (P < 0.01). There was no significant difference in TIMP1 mRNA levels between IFNgamma groups and controls.
CONCLUSIONIFNgamma suppresses expression of Col I and Col III whereas it has no effect on TIMP1 mRNA expression. The antifibrotic mechanism of IFNgamma may be partly due to its down-regulation of Col I and Col III mRNA levels in HSC-T6 cells.
