Study of the down-regulating effect of hepatitis C virus NS5A protein on NACA promoter.
- Author:
Jiu-zeng DAI
1
;
Jun CHENG
;
Jian-hui QU
;
Dong JI
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Down-Regulation; Humans; Molecular Chaperones; biosynthesis; genetics; Molecular Sequence Data; Promoter Regions, Genetic; genetics; Transcriptional Activation; Transfection; Viral Nonstructural Proteins; genetics; pharmacology
- From: Chinese Journal of Hepatology 2005;13(8):579-581
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of hepatitis C virus (HCV) non-structure protein NS5A on the activity of calcium-regulating protein alpha subunit of nascent polypeptide-associated complex (NACA) promoter.
METHODSHepG2 cell plasmid pCAT3-NACA, containing NACA promoter, was transfected alone or cotransfected with pcDNA3.1(-)-NS5A, and chloramphenicol acetyl transferase (CAT) enzyme activity was assayed by enzyme-linked immunoassay (ELISA).
RESULTSThe CAT activity in the pcDNA3.1(-)-NS5A cotransfection group was 20.7% of the CAT activity in the pCAT3-NACA group.
CONCLUSIONHCV non-structural protein NS5A has a down-regulating effect on the promoter of NACA gene.
