Molecular epidemiology related to human immunodeficiency virus type 1 infection in men having sex with men in Xi'an
10.3760/cma.j.issn.0254-6450.2014.04.017
- VernacularTitle:西安市男男性行为人群感染HIV-1的分子流行病学研究
- Author:
Hailan ZHANG
1
,
2
;
Yongping YAN
;
Hengxin LI
;
Duan LI
;
Xiaoli WEI
;
Haichao ZHENG
;
Xin ZHAO
Author Information
1. 710032西安,第四军医大学军事预防医学系流行病学教研室
2. 西安市疾病预防控制中心
- Keywords:
Men having sex with men;
AIDS;
HIV-1 subtype
- From:
Chinese Journal of Epidemiology
2014;35(4):421-424
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the subtype distribution of HIV-1 infection among men having sex with men (MSM) in Xi' an city.Methods 5 ml anti-coagulating blood samples were collected from MSM who had been reported during 2010-2012 in Xi'an.Both gag and env genes were amplified by nested RT-PCR from the extracted RNA and then sequenced.The acquired sequences were compared with international subtype references,and then the genetic distances were calculated and phylogenetic trees were constructed by Mega 5.2.Epidemiological information including sexual behavior characteristics,history of drug use,blood donation,etc.were gathered.Results 168 samples were successfully amplified and sequenced.Results from Phylogenic analysis showed that the gag and env sequences of 165 samples shared the same subtypes,of which 79 (47.0％) were CRF01_AE,74 (44.0％) were CRF07_BC and 12 (7.1％) belonged to subtype B.There were 3 samples with gag and env sequences classified into different subtypes,of which 2 (1.2％) were CRF01_AE/A1,and the other 1 was CRF07_BC/CRF01_AE.Conclusion At least three HIV-1 subtypes including CRF01_AE,CRF07_BC were identified among MSM population in Xi'an city.
