Phenotypic diversity of toxigenic Vibrio cholerae O1 El Tor strains identified in China.

Xuan ZHAO; Li ZHANG; Jie LI; Biao KAN; Weili LIANG

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Phenotypic diversity of toxigenic Vibrio cholerae O1 El Tor strains identified in China.

Author: Xuan ZHAO ^{1
,

2} ; Li ZHANG ³ ; Jie LI ³ ; Biao KAN ³ ; Weili LIANG ⁴
Author Information

1. State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
2. Tianjin Centre for Disease Control and Prevention.
3. State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
4. State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. Email: liangweili@icdc.cn.
Publication Type:Journal Article
MeSH: China; Genotype; Phenotype; Polymorphism, Genetic; Vibrio cholerae O1; genetics
From: Chinese Journal of Epidemiology 2014;35(5):573-575
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo understand the phenotypic diversity of toxigenic Vibrio cholerae O1 El Tor strains isolated from different provinces in China during the last 50 years.
METHODSTraditional biotyping testings including susceptibility to polymyxin B, sensitivity to group IV phage, Voges-Proskauer test and haemolysis of sheep erythrocytes were conducted.
RESULTSData from Biotype-specific phenotype analysis revealed that only 133 isolates carried the typical El Tor phenotypes while the other 251 isolates displayed atypical El Tor phenotypes. Combined with ctxB, rstR genotypes and phenotypic characteristics, 64 isolates were identified as typical El Tor biotype, 21 were El Tor variants that showing the typical El Tor biotype-specific phenotype but with ctxB(class). 280 isolates were defined as the hybrid groups with traits of both classical and El Tor biotypes that could be further classified into 45 groups, based on the combination of genotypes of ctxB, rstR and phenotypic characteristics.
CONCLUSIONToxigenic Vibrio cholerae O1 El Tor strains that isolated from different provinces in China displayed high phenotypic diversity. The traditional biotype traits could not be used to correctly distinguish the two different biotypes.