Analyses of exercise-induced muscle damage-specific microRNA expression and molecular target of sarcolemmal damage in rats.
- Author:
Yu-Ming XU
1
;
Jian-Min CAO
2
;
Jun-Ping LI
2
;
Qiao-Ting HUANG
2
;
Ping WANG
3
Author Information
1. Sports and Health College of Hangzhou Normal University, Hangzhou 310036, China. xuyuming110@126.com.
2. Sport Science College of Beijing Sport University, Beijing 100086, China.
3. Sports and Health College of Hangzhou Normal University, Hangzhou 310036, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Dystrophin;
genetics;
MAP Kinase Kinase 4;
genetics;
MAP Kinase Signaling System;
Male;
MicroRNAs;
genetics;
Physical Conditioning, Animal;
adverse effects;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Real-Time Polymerase Chain Reaction;
Running;
Sarcolemma;
pathology;
Utrophin;
genetics
- From:
Acta Physiologica Sinica
2017;69(3):276-284
- CountryChina
- Language:Chinese
-
Abstract:
In the present study, we were to screen the specific microRNA (miRNA) of exercise-induced muscle damage (EIMD) and assess the EIMD-specific miRNAs-regulated target of sarcolemmal damage in rats. Twenty-four male Sprague-Dawley (SD) rats were randomly divided into 3 groups, which included sedentary (C), 24 h post-exercise (E24) and 48 h post-exercise (E48) groups. Rat EIMD model was established by an acute eccentric exercise, i.e., a downhill running treatment at -16º gradient. EIMD characteristics were verified by Evans blue dye staining, differentially expressed miRNAs were detected by microarray assay, EIMD-specific miRNAs expressions were further validated by real-time quantitative RT-PCR (RT-qPCR), and targets of the miRNAs were predicted based on mRNA expressions of associated proteins and related pathway core molecules of sarcolemmal damage. Two EIMD-specific expressed miRNAs, including miR-206-3p and miR-139-3p, were found in the study. There was a significantly negative correlation (P < 0.05) between miR-206-3p expression and dystrophin (r = -0.68), utrophin (r = -0.64), JNK (r = -0.62) or ERK1 (r = -0.68) respectively, but no correlation was found between miR-139-3p and these biomolecules. The results suggest that: i) the expression profile of miRNAs in rat is significantly affected by EIMD, ii) miR-206-3p and miR-139-3p are the EIMD-specific miRNAs, and iii) miR-206-3p may control sarcolemmal damage by regulating dystrophin, utrophin, JNK and ERK1.
