A study on toxic effects of sodium salicylate on rat cochlear spiral ganglion neurons: dopamine receptors mediate expressions of NMDA and GABAreceptors.
- Author:
Ting-Jia WEI
1
;
Hui-Ying CHEN
1
;
Xi HUANG
1
;
Jing-Jin WENG
1
;
Jiang-Yuan QIN
1
;
Ji-Ping SU
2
Author Information
1. Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
2. Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China. ymsu2@126.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Benzazepines;
pharmacology;
Cells, Cultured;
Cochlea;
cytology;
Neurons;
drug effects;
Rats;
Receptors, Dopamine;
metabolism;
Receptors, GABA-A;
metabolism;
Receptors, N-Methyl-D-Aspartate;
metabolism;
Sodium Salicylate;
toxicity;
Spiral Ganglion;
drug effects
- From:
Acta Physiologica Sinica
2017;69(3):285-290
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the present study was to observe whether dopamine receptor (DR) was involved in the effects of sodium salicylate (SS) on the expressions of N-methyl-D-aspartic acid (NMDA) and γ-aminobutyric acid (GABA) receptors in rat cochlear spiral ganglion neurons (SGNs). Forty-eight hours after primary culture of rat SGNs, immunofluorescence technique was applied to detect expressions of DR1 and DR2, the two subtypes of dopamine receptors. Western blot was performed to assess NMDA receptor NR1 subunit and GABAreceptor subunit α2 (GABRα2) protein expressions in the SGNs after the treatments of SS alone or in combination with DR antagonists. The results demonstrated that: (1) The DR1 and DR2 were expressed in the bodies and axons of the SGN; (2) After the treatment with SS, the surface protein expressions of GABRα2 and NR1 were decreased by 44.69% and 21.57%, respectively, while the total protein expressions showed no significant changes; (3) Neither SS + SCH23390 (DR1 antagonist) group nor SS + Eticlopride (DR2 antagonist) group showed significant differences in GABRα2 and NR1 surface protein expressions compared with the control group. These results suggest that SS regulates the surface GABAand NMDA receptors trafficking on SGN, and the mechanism may involve DR mediation.
