Expression of annexin I in human pancreatic cancer and the influence of its down-regulation on biology of this cancer.
- Author:
Qian LIU
1
;
Cheng-Feng WANG
;
Zhuan ZHOU
;
Hai HU
;
Dong-Bing ZHAO
;
Xiao-Guang NI
;
Xiao-Feng BAI
;
Ji-Dong GAO
;
Yan-Tao TIAN
;
Ping ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Annexin A1; genetics; metabolism; Apoptosis; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Down-Regulation; Gene Expression Regulation, Neoplastic; Genetic Vectors; Humans; Pancreatic Neoplasms; genetics; metabolism; pathology; RNA Interference; RNA, Messenger; metabolism; RNA, Small Interfering; genetics; Transfection
- From: Chinese Journal of Oncology 2007;29(10):738-741
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of annexin in human pancreatic cancer and to elucidate its role in oncogenesis of pancreatic cancer.
METHODSA pancreatic carcinoma cell line Suit-II with high-expression of annexin I gene was adopted. Three subtypes of annexin I -siRNA sequences and a non-related fragment were combined, and the eukaryotic expression vectors bearing siRNA fragments were constructed. Then they were transfected into pancreatic carcinoma cells to knock down the expression of annexin I by RNAi. After knocking down the expression of annexin I , the growth speed, cell cycling, morphological features and apoptosis of pancreatic carcinoma cells were examined by RT-PCR and MTT test.
RESULTSWhen the expression of annexin I was blocked, the growth speed of pancreatic carcinoma cells was significantly decreased, the morphological features were changed and pronounced apoptosis occurred.
CONCLUSIONAnnexin I can modulate pancreatic carcinoma cell cycle, promote the cell proliferation, increasingly stimulate the cell growth, and suppress the process of apoptosis in pancreatic carcinoma cells.
