Expression of nucleostemin mRNA and protein in the esophageal squamous cell carcinoma.
- Author:
Gong-Yuan ZHANG
1
;
Lei YIN
;
Sheng-Lei LI
;
Wen-Ying XING
;
Qiu-Min ZHAO
;
Xiao-Ping LE
;
Dong-Ling GAO
;
Kui-Sheng CHEN
;
Yun-Han ZHANG
;
Qin-Xian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; metabolism; pathology; Carrier Proteins; biosynthesis; genetics; Esophageal Neoplasms; metabolism; pathology; Esophagus; pathology; Female; GTP-Binding Proteins; Gene Expression Regulation, Neoplastic; Humans; Hyperplasia; Lymphatic Metastasis; Male; Middle Aged; Mucous Membrane; metabolism; Neoplasm Invasiveness; Neoplasm Staging; Nuclear Proteins; biosynthesis; genetics; Precancerous Conditions; metabolism; pathology; RNA, Messenger; metabolism
- From: Chinese Journal of Oncology 2008;30(2):125-128
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mRNA and protein expression of nucleostemin (NS) in human esophageal squamous cell carcinoma.
METHODSThe mRNA and protein expression of NS were detected in 31 mucosal atypical hyperplasia specimens, 62 esophageal squamous cell carcinoma specimens and the matched normal esophageal mucosa samples by RT-PCR and immunohistochemistry method, respectively.
RESULTSThe positive expression rate of NS protein in normal esophageal mucosa, atypical hyperplasia and esophageal squamous cell carcinoma was 17.7% (11/62), 41.9% (13/31) and 69.4% (43/62), respectively. There was a significant difference among the above three groups (chi2 = 33.676, P < 0.01). The expression levels of NS mRNA in esophageal squamous cell carcinoma (0.971 +/- 0.121) was significantly higher than that in the atypical hyperplasia (0.913 +/- 0.085) and also in the normal esophageal mucosa (0.866 +/- 0.103; F = 14.829, P < 0.01). The expression level of both NS protein and mRNA was positively correlated with histological grade, infiltration depth, and lymph node metastasis (P < 0.05), but not with age, gender or pathological type (P > 0.05).
CONCLUSIONOur results indicate that nucleostemin mRNA and protein are over-expressed in human esophageal squamous cell carcinoma, and it may be related with its oncogenesis.
