Biological effect of endostatin on transplanted human lung adenocarcinoma Calu-6 tumor in nude mice.
- Author:
Jing WANG
1
;
Chun HUANG
;
Xi-Yin WEI
;
Zhong-Li ZHAN
;
Hui SUN
;
Yi YANG
;
Kai LI
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; pathology; Angiogenesis Inhibitors; administration & dosage; pharmacology; Animals; Antigens, CD; metabolism; Antineoplastic Agents; administration & dosage; pharmacology; CD146 Antigen; metabolism; Cell Line, Tumor; Cyclooxygenase 2; metabolism; Dose-Response Relationship, Drug; Endoglin; Endostatins; administration & dosage; pharmacology; Endothelial Cells; pathology; Female; Humans; Inhibitor of Apoptosis Proteins; Lung Neoplasms; metabolism; pathology; Mice; Mice, Inbred BALB C; Mice, Nude; Microtubule-Associated Proteins; metabolism; Microvessels; pathology; Neoplasm Transplantation; RNA, Messenger; metabolism; Random Allocation; Receptors, Cell Surface; metabolism; Tumor Burden; drug effects; Vascular Endothelial Growth Factor A; metabolism
- From: Chinese Journal of Oncology 2008;30(4):266-269
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the effect of endostatin on growth and neoplastic angiogenesis in transplanted human lung adenocarcinoma Calu-6 tumor in nude mice.
METHODSTo treat Calu-6 tumor-bearing mice with endostatin at different doses, and to record the changes of the tumor size. The expressions of survivin, VEGF, COX-2 and MVD in tumor tissue were examined by immunohistochemistry staining, circulating endothelial cells (CECs) by flow cytometry and mRNA of CD146 and CD105 by RT-PCR and real-time PCR.
RESULTSAfter endostatin treatment, the tumor size was conspicuously shrunk, and the expressions of survivin, COX-2 and VEGF protein and MVD in tumor tissue decreased concomitantly with the significant difference between each of trial groups and control group (all P < 0.05). Both CECs and mRNA of CD146 and CD105 diminished remarkably. A positive correlation between both exhibition and change of amount of activated CECs and survivin, VEGF expression and MVD count in tumor tissue was found.
CONCLUSIONEndostatin can decrease the expression of survivin, COX-2, VEGF and MVD, and to inhibit the growth of transplanted tumor. Activated CECs may probably serve as an ideal marker to predict the efficacy and prognosis of anti-angiogenesis therapy.
