Expression and clinical significance of HMGB1 and RAGE in cervical squamous cell carcinoma.
- Author:
Quan HAO
1
;
Xiao-Qin DU
;
Xin FU
;
Jing TIAN
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Carcinoma in Situ; metabolism; pathology; Carcinoma, Squamous Cell; metabolism; pathology; Cervix Uteri; metabolism; pathology; Female; Gene Expression Regulation, Neoplastic; HMGB Proteins; metabolism; Humans; Immunohistochemistry; Lymphatic Metastasis; Neoplasm Invasiveness; Neoplasm Metastasis; Neoplasm Staging; RNA, Messenger; metabolism; Receptor for Advanced Glycation End Products; Receptors, Immunologic; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Uterine Cervical Neoplasms; metabolism; pathology
- From: Chinese Journal of Oncology 2008;30(4):292-295
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association between high mobility group box1 (HMGB1) and cervical squamous cell carcinoma (CSCC), and explore the role of HMGB1/RAGE pathway in the metastasis of CSCC.
METHODSLevels of HMGB1 mRNA and RAGE mRNA in CSCC and normal cervical tissues were detected by real time quantitative polymerase chain reaction (qRT-PCR), and the level of HMGB1 protein was determined by immunohistochemistry and Western blotting.
RESULTSThe mRNA and protein expression of HMGB1 was significantly higher in CSCC than that in normal cervical tissue (P < 0.05), correlated with stage, invasion and metastasis (P < 0.05), but not with tumor size and differentiation (P > 0.05). The levels of RAGE mRNA and HMGB1 mRNA were both significantly higher (r = 0.663, P < 0.05) in metastatic CSCC in comparison with those in the non-metastatic cases.
CONCLUSIONHMGB1 is involved in the invasion and metastasis of CSCC, and HMGB1/RAGE pathway plays an important role in the metastasis of CSCC.
