Expression variation and significance of Skp2 and p27(kip1) during the proliferation of Jurkat cells.
- Author:
Jian-Xin LU
1
;
Yu-Chan WANG
;
Ai-Guo SHEN
;
Yue-Ming ZHAO
;
Cheng-Long SUN
;
Dong-Mei ZHANG
;
Chun CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Nucleus; metabolism; Cell Proliferation; Cyclin-Dependent Kinase Inhibitor p27; metabolism; Cytoplasm; metabolism; Humans; Jurkat Cells; Lymphoma, B-Cell; metabolism; pathology; Protein Binding; S-Phase Kinase-Associated Proteins; metabolism
- From: Chinese Journal of Oncology 2008;30(5):330-334
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression variation and significance of Skp2 and p27(kip1) during the proliferation of lymphoma cell line Jurkat cells.
METHODSThe binding of p27(kip1) and Skp2 in Jurkat cells were detected by immunoprecipitation. Jurkat cells were treated with serum starvation and release synchronization. The expression variation and subcellular localization of p27(kip1) and Skp2 were detected by subcellular fractionation, Western blot and double immunofluorescence labelling.
RESULTSThe results of immunoprecipitation suggested that p27(kip1) and Skp2 could bind each other in Jurkat cells. During the proliferation of Jurkat cells, the protein expression of p27(kip1) decreased and intranuclear p27(kip1) decreased significantly, while the Skp2 protein increased and cytoplasmic Skp2 increased significantly.
CONCLUSIONDuring the proliferation of Jurkat cells, the increased cytoplasmic synthesis of Skp2 may speed up p27(kip1) degradation via the ubiquitin-proteasome pathway, then intranuclear p27(kip1) decreases significantly, leading to an increased cell cycling activity.
