Effect of focal adhesion kinase on biological characteristics of Tca8113 tongue squamous cell carcinoma.
- Author:
Mei-xian FANG
1
;
Hua-wei HE
;
Dan WANG
;
Zhi-xiu HE
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; Cell Line, Tumor; Down-Regulation; Focal Adhesion Protein-Tyrosine Kinases; Humans; Tongue Neoplasms; Transfection
- From: West China Journal of Stomatology 2007;25(2):115-117
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of FAK on biological characteristics of tongue squamous cell carcinoma cell Tca8113.
METHODSLipofect was used to transfect FAK into Tca8113 cell lines. The difference in expression of FAK between the transfected and non-transfected cell lines was detected by immunofluorescence assay. Then by use of transwell room and wash way, the difference in invasion and adhesion ability between the transfected and non-transfected cell lines was tested. MTT method was adopted in finding the change of proliferation.
RESULTSRT-PCR analysis showed that the FAK gene was downregulated by Tca8113 cells. FAK expression was significantly down regulated by the transfection of FAK ASODN (P<0.05). Antisense FAK ODN significantly inhibited proliferation, adhesion and the invasion of Tca8113 cells.
CONCLUSIONFAK plays an important role on biological characteristics of tongue squamous cell carcinoma cell Tca8113.
