Nasal immunization with co-expression plasmid harboring genes encoding porphyromonas gingivalis FimA and human interleukin-15 in mice.
- Author:
Guang-shui JIANG
1
;
Hong-mei GUO
;
Xi-jun WANG
;
Pi-shan YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Humans; Immunization; Interleukin-15; Mice; Mice, Inbred BALB C; Plasmids; Porphyromonas gingivalis
- From: West China Journal of Stomatology 2007;25(2):177-179
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the antibody responses induced by recombinant plasmid plRES-fimA:IL15 via nasal immunization to BABL/c mice and the regulation of IL-15 to sIgA.
METHODSBABL/c mice were immunized with recombinant plasmids pIRES-fimA:IL15 and pIRES-fimA via nasal or intramuscular route. Serum IgG and salivary sIgA levels after immunization were analyzed by ELISA.
RESULTSNasal immunization with plasmids pIRESfimA:IL15 or pIRES-fimA elicited significant higher level of salivary FimA-specific sIgA responses compared with intramuscular immunization. There was no significant difference of the serum IgG responses between nasal immunization mice and intramuscular immunization mice. Nasal immunization with plasmid pIRES -fimA:IL15 elicited significant higher level of salivary sIgA response than with pIRES-fimA (P<0.05).
CONCLUSIONNasal dropping may be an effective mucosal immunization route of anti-Porphyromonas gingivalis DNA vaccine to elicit specific antibody responses in serum and oral region. IL-15 has a positive regulation effect to sIgA response.
