Induction of drug resistance in Tca8113 cell line by exposing to chemotherapy drug.

Author: Ge FENG ¹ ; Da-zhang WANG ; Huai-qing CHEN ; Jia HE ; Weidong LENG
Author Information

1. Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Publication Type:Journal Article
MeSH: Antineoplastic Agents; Cell Line; Cell Line, Tumor; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Humans
From: West China Journal of Stomatology 2007;25(2):184-187
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the drug resistance changes in Tca8113 cell lines by exposing to carboplatin.
METHODSThe concentration of carboplatin added to Tca8113 cells was increased gradually and continually, which was to induce the carhoplatin-resistance in Tca8113 cells. The sensibility to drugs of the cells was analyzed by MTT method. Immunocytochemistry and RT-PCR were utilized to examine the expression of multidrug resistance proteins and genes.
RESULTSAfter exposing to carboplatin, the Tca8113/CBP cells had higher drug-resistance to CBP, MTX, PYM, VCR and higher expression of MRP, GST-pi than Tca8113 cells.
CONCLUSIONMultidrug resistance of Tca8113/CBP is associated with over expression of MRP, GST-pi and MDR. Tca8113/CBP can provide an ideal model for multidrug resistance research.