Effects of retinoic acid on differentiation of periodontal ligament cells.

Author: Yan WANG ¹ ; Pi-Shan YANG ; Ming-wen FAN
Author Information

1. Dept. of Endodontics, College of Stomatology, Shandong University, Jinan 250012, China.
Publication Type:Journal Article
MeSH: Cell Differentiation; Fibroblasts; Gingiva; Osteoblasts; Periodontal Ligament; RNA, Messenger; Tretinoin
From: West China Journal of Stomatology 2007;25(2):198-201
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effect of retinoic acid on differentiation of periodontal ligament cells (PDLCs) and gingival fibroblasts (GFs).
METHODSThe periodontal ligament cells and gingival fibroblasts were cultured, challenged with different concentrations of retinoic acid in medium and detected for the alkaline phosphatase (ALP) activity and its mRNA by biochemical technique, in situ hybridization and RT-PCR.
RESULTSALP activity in normal PDLCs was higher than that in GFs. The mRNA signals were positive in PDLCs, and negative in GFs. After treated with different concentrations of retinoic acid, ALP activity of PDLCs was increased than that of the control, and its mRNA signals were enhanced, especially in 5 x 10(-6) mol/L. The treated GFs showed a slight increase of ALP activity and a weak band of mRNA signals only in 5 x 10(-6) molUL concentration.
CONCLUSIONThere were differences between PDLCs and GFs in differentiating into osteoblast-like cells.