Targeting transgene effect of adTERT-TRAIL on salivary adenoid cystic carcinoma cell line SACC-83.
- Author:
Tao SU
1
;
Hong-Chen SUN
;
Chang-Yu ZHENG
;
Hong ZHANG
;
Dong-min LU
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Adenoid Cystic; Cell Line; Cell Line, Tumor; Genetic Vectors; Humans; Promoter Regions, Genetic; Telomerase; Transfection; Transgenes
- From: West China Journal of Stomatology 2007;25(2):202-205
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the targeting expression of TRAIL gene driven by human telomerase reverse transcriptase (hTERT) promoter in SACC-83 cell with telomerase activity.
METHODSAdenovirus vector AdTERT-TRAIL was constructed by homologus recombination. After transfecting AdTERT-TRAIL into SACC-83 cell and HEL cell, its effect on these cells in vitro was investigated using RT-PCR technique, MTT method and flow cytometry.
RESULTSAfter transfection of AdTERT-TRAIL, expression of extrinsic TRAIL gene driven was detected in SACC-83, the proliferation of SACC-83 cell showed significant inhibitory effect (the relative cell viability was 49.70%) and its apoptotic rate was promoted (30.49%), whereas no TRAIL gene was detected in HEL cell, also no inhibitory effect was observed in HEL cell and its apoptotic rate showed little change.
CONCLUSIONAdenovirus vector AdTERT-TRAIL was successfully constructed, which can be used to induce expression of TRAIL gene in SACC-83 cell with targeting effect.
