Initial study on discrimination of oral microorganisms with the metabonomics technique.
- Author:
Miao LI
1
;
Li-ying XIAO
;
Ji-yao LI
;
Xiang-li KONG
;
Jia-hui YU
;
Jing-lin ZHOU
;
Xiao-rong XIAO
;
Zhu ZHU
;
Qi-mei GONG
;
Wei LI
Author Information
- Publication Type:Journal Article
- MeSH: Culture Media; Magnetic Resonance Spectroscopy; Metabolomics; Streptococcus mutans
- From: West China Journal of Stomatology 2007;25(4):342-344
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the feasibility of employing metabonomics method in identification of oral pathogenic bacteria.
METHODSThe Streptococcus mutans ATCC25175 and Actinomyces viscosus ATCC15987 were respectively inoculated in same certain culture medium. The growth curves of the inoculated bacteria were drown by turbidimetry. The culture solutions in four different growth phases of the both bacteria were used to test with the 1H-Nuclear magnetic resonance (1H-NMR) spectroscopy respectively. The data of 1H-NMR spectroscopy results were analyzed by principal components analysis (PCA).
RESULTSThe PCA showed the obvious clustering phenomena and the points of two group data stayed differentially together by two clusters. Therefore, the NMR-based metabonomics profiles can discriminate the two different kind of bacteria.
CONCLUSIONThe metabonomics can be expected to be a kind of promising useful method in quick discrimination of oral pathogenic bacteria.