Effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in polarized osteoclasts.
- Author:
Qing-hong ZHANG
1
;
Xing LIANG
;
Meng-tao LIU
;
Bao-min ZHU
;
Jun FU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Osteoclasts; RNA, Messenger; Rats; Shear Strength; Stress, Mechanical
- From: West China Journal of Stomatology 2007;25(4):396-398
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in rat polarized osteoclasts.
METHODSRat polarized osteoclasts suffered 0.0 (control group), 0.9, 2.9, 8.7 and 26.3 dynes/cm2 fluid shear stress for 30 min. mRNA expression of ATP6V1a1 was detected by Real-Time fluorescent quantitation PCR.
RESULTSmRNA expression of ATP6V1a1 in 0.0 (control group), 0.9, 2.9, 8.7 and 26.3 dynes/cm2 groups was (1.14 +/- 0.06) x 10(6), (1.62 +/- 0.09) x 10(6), (2.28 +/- 0.13) x 10(6), (3.24 +/- 0.18) x 10(6), (9.16 +/- 0.53) x 10(6) copy numbers, respectively (P < 0.05).
CONCLUSIONIn the present study, polarized osteoclasts are sensitive to fluid shear stress. mRNA expression of ATP6V1a1 has increscent tendency along with increasing of fluid shear stress strength.
