Fluid shear stress increases the Ca2+ concentration in bone-marrow derived osteoclast-like cells.
- Author:
Ming CHEN
1
;
Xing LIANG
;
Ying WEN
;
Bao-jing BAI
;
Meng-lu HUANG
;
Wei-min GAO
Author Information
- Publication Type:Journal Article
- MeSH: Aniline Compounds; Bone Marrow; Bone Marrow Cells; Calcium; Cells, Cultured; Osteoclasts; Stress, Mechanical; Xanthenes
- From: West China Journal of Stomatology 2007;25(4):412-414
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the change of Ca2+ density in cultured osteoclast-like cells in response to fluid shear stress.
METHODSLaser scanning confocal microscope and fluorescent probe were used to detect the free Ca2+ in osteoclast-like cells before and after undergoing fluid shear stress. The images were analyzed and compared with image software.
RESULTSAt 37 degrees C the free Ca2+ in osteoclast-like cells could be labelled effectively with 10 micromol/L Fluo-3/AM. Compared with contol group, the average intensity of Ca2+ fluorescent signal in osteoclast-like cells undergoing fluid shear stress increased significantly.
CONCLUSIONThe Cal2+ concentration in bone-marrow derived osteoclast-like cells is sensitive to fluid shear stress, which suggests osteoclast-like cells modulate their function in response to fluid shear stress through the change of free Ca2+ concentration.
