Cloning of human bone morphogenetic protein-2 gene and the construction of its eukaryotic expression vector.
- Author:
Nuo ZHOU
1
;
Xuan-ping HUANG
;
Ni LIAO
;
Shan-liang WEI
;
Fei-xin LIANG
;
Hua-ming MAI
Author Information
- Publication Type:Journal Article
- MeSH: Bone Morphogenetic Protein 2; Cloning, Organism; Genetic Therapy; Genetic Vectors; Humans; Polymerase Chain Reaction; Sequence Analysis, DNA; Transfection
- From: West China Journal of Stomatology 2007;25(5):487-489
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clone human bone morphogenetic protein-2 (hBMP2) gene and construct its eukaryotic expression vector pcDNA3.1 -hBMP2.
METHODSHuman BMP2 gene was amplified by RT-PCR method from human osteosarcoma cells and constructed into eukaryotic expression vector pcDNA3.1-hBMP2. The gene in the vector pcDNA3.1-hBMP2 was identified by PCR amplification, enzyme digestion and DNA sequencing.
RESULTSThe cloned DNA was confirmed to be hBMP-2 gene.
CONCLUSIONIn this study, hBMP2 gene is successfully cloned and its eukaryotic expression vector pcDNA3.1-hBMP2 is constructed, which provides the foundation of using BMP2 gene therapy to accelerate new bone formation in distraction osteogenesis.
