Ectopic bone induction in vivo after transplantation of skeletal satellite cells from green fluorescence protein transgenic mouse transfected by adenoviral vectors encoding bone morphogenetic protein-2.
- Author:
Feng-he ZHANG
1
;
Ping HUANG
;
Feng-cai WEI
;
Shu-yang SUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins; Bone and Bones; Cell Differentiation; Fluorescence; Genetic Vectors; Green Fluorescent Proteins; Mice; Mice, Transgenic; Myoblasts; Osteoblasts; Transfection; Transforming Growth Factor beta
- From: West China Journal of Stomatology 2007;25(5):504-507
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the ability of induced ectopic bone using skeletal muscles satellite cells (SMSCs) from newborn green fluorescence protein (GFP) transgenic mice mediated by Ad-BMP2.
METHODSTransplantation of SMSCs transduced with Ad-BMP2 into back lamb muscles of subfascia in wildtype 129sv mice with a complex of collagen scaffords, then the tissue histologic examination, X ray plain film, fluorescence microscopy were used.
RESULTSTransplantation of SMSCs transfected with Ad-BMP2 into back lamb muscles of subfascia generated ectopic bone formation involving GFP-positive osteoblasts and osteocytes 2 weeks and mature bone formation 4 weeks after transplantation. SMSCs non-transfected with Ad-BMP2 failed to induce ectopic bone formation.
CONCLUSIONSMSCs retain differentiation potentitality into osteoblasts in response to Ad-BMP2. They are useful tools for analyzing the process of osteoblast differentiation in vivo after transplantation.
