Expression of VEGFA, VEGFC, angiopoietin-1, angiopoietin-2 and their receptors on development liver during gestation of weeks 3-12 of human embryo.
- Author:
Yan MEI
1
;
Ai-Dong LI
;
Ji-Ying JIANG
;
Hong-Ying ZHOU
;
Hui-Jun YANG
;
Shu-Xia YANG
;
Hua-Rong HONG
;
Hong-Rui SONG
Author Information
1. Center of Scientific Research and Experimentation of Chengdu Military Medical College, The Third Military Medical University, Chengdu 610083, China.
- Publication Type:Journal Article
- MeSH:
Angiopoietin-1;
analysis;
Angiopoietin-2;
analysis;
Extracellular Matrix Proteins;
analysis;
Female;
Gestational Age;
Humans;
Immunohistochemistry;
Liver;
chemistry;
embryology;
Pregnancy;
Receptor, TIE-2;
analysis;
Vascular Endothelial Growth Factor A;
analysis;
Vascular Endothelial Growth Factor C;
analysis;
Vascular Endothelial Growth Factor Receptor-1;
Vascular Endothelial Growth Factor Receptor-3;
analysis
- From:
Journal of Experimental Hematology
2004;12(5):632-636
- CountryChina
- Language:Chinese
-
Abstract:
The aim was to study the expression of VEGF-A, VEGF-C, angiopoietin-1, angiopoietin-2 and their receptors on development liver during gestation of weeks 3-12 of human embryo. Human embryo contingently aborted at 3-12 weeks of gestation were collected with signed agreements of the pregnant women suffered from accidental abortions. The specimens were fixed by 4% paraformaldehyde and embedded by paraffin. 5 microm serial sections were made. HE staining, immunohistochemistry method and light-microscope were employed. The results showed that at 4-5 weeks of development, liver was constituted by a few hepatic cords. Hematopoietic cell or blood cells were undetectable in the 4 week of gestation. A few cells which were larger, rounded and nucleared cells appeared and expressed VEGFA, flt-4 and Tie-2 proteins strongly in liver at 5 weeks of gestation. The number of these immuno-positive cells was highest in the 7th week and decreased at 11-12 weeks of gestation. These cells expressed flk-1 transiently in the 6th week. VEGF-C and flt-1 were expressed by hepatic cells from weeks 7 to 12 of gestation. The immuno-positive products were deposited in plasma of hepatic cells. Angiopoietin-1, angiopoietin-2 and Tie-2 were detectable on those cells which expressed VEGFA, flt-4 and Tie-2 from weeks 5 to 12 of gestation. The expression of angiopoietin-1 and angiopoietin-2 were weakly and Tie-2 was strongly. They were expressed weakly too by hepatic cells at 5 to 12 weeks of gestation. All factors and their receptors were undetectable on vascular endothelial cells at 4-12 weeks of gestation. It is concluded that the expression patterns of VEGF family on cells of liver are different before and after 7 weeks of gestation. The hematopoiesis in fetal liver may be related to development of hepatic cell.
