Study on the expression of hematopoietic growth factor gene in human umbilical vein endothelial cells using gene chip.
- Author:
Zhao-Dong ZHONG
1
;
Ping ZOU
;
Ling-Bo LIU
;
Yong YOU
;
Shiang HUANG
Author Information
1. Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Endothelial Cells;
metabolism;
Gene Expression Profiling;
Hematopoietic Cell Growth Factors;
genetics;
Humans;
Oligonucleotide Array Sequence Analysis;
Umbilical Veins;
metabolism
- From:
Journal of Experimental Hematology
2004;12(5):637-639
- CountryChina
- Language:Chinese
-
Abstract:
In order to detect the hematopoietic growth factor gene expressed in human umbilical vein endothelial cells using gene chip, human umbilical vein endothelial cells (ECV304) were cultured in vitro and divided into VEGF group and control group in same medium. 50 ng/ml hVEGF165 was added in the VEGF group. After culture for 24 hours all cells were collected for total RNA extraction. Then, cDNAs were marked with Cy3 and Cy5 for control group and VEGF group, respectively, and hybridized with gene chip. Hybridization signals were collected and analyzed following scanning by laser co-focal microscopy. The results showed that a large number of hematopoietic growth factor and receptor genes (Epo/R, GM-CSF/R, G-CSF/R, LIF, IL-3, TPO, Flt-3, SCF) were expressed in both groups, while many other growth factors (VEGF, IGF2, PDGFA, PDGFB, TGFbeta1) and receptors (neuropilin-1, neuropilin-2, TGFbeta-R1)were expressed. The differentially expressed genes amounted to 24. It is concluded that many hematopoietic growth factors and receptors expressed by hUVECs could be analyzed in a short period by using gene chip, which provides a powerful method for further studies on characteristics of vascular endothelial cells.
