Inhibition effect of bone marrow mesenchymal stem cells on T-lymphocyte proliferation through up-regulation of CD8+CD28- T cells.
- Author:
Wei ZHANG
1
;
Wei GE
;
Chang-Hong LI
;
Sheng-Guo YOU
;
Qin HAN
;
Wei-Min DENG
;
Chun-Hua ZHAO
Author Information
1. State Key Laboratory of Experimental Hematology, Institute of Hematology, Chenese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
physiology;
CD28 Antigens;
analysis;
CD8 Antigens;
analysis;
Humans;
Lymphocyte Activation;
Mesenchymal Stromal Cells;
physiology;
T-Lymphocyte Subsets;
immunology;
Up-Regulation
- From:
Journal of Experimental Hematology
2004;12(5):666-669
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explore effect of CD8+CD28- T-lymphocyte in the inhibition of mesenchymal stem cells (MSC) on T-lymphocyte proliferation. T cells were harvested by using nylon column and CD8+ T cells were sorted by magnetic beads; the T-lymphocyte proliferation in the presence of PHA was evaluated by MTT; the proportion of CD8+CD28- T cells was assayed by fluorescence-activated cell sorter (FACS). The results showed that MSC inhibited T-lymphocyte proliferation and the inhibitory effect depended on the amount of MSC; the data of FACS indicated that in the CD8+ T cells co-cultured with MSC, CD8+CD28- T cells were up-regulated significantly, compared with the non-treated CD8+ T cells. In conclusion, MSC perform their immunosuppressive function by up-regulation of CD8+CD28- T cells.
