Comparison of platelet activators used in slide platelet aggregation test.
- Author:
Xi-Lin OUYANG
1
;
Jing-Han LIU
;
Gui-Xiang SUN
;
Xi-Jin LI
;
Zi-Lin LIN
;
Da-Yong GAO
Author Information
1. Department of Blood Transfusion, General Hospital of PLA, Centre for Clinic Blood Transfusion Medicine of PLA, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Cyclic AMP;
pharmacology;
Edetic Acid;
pharmacology;
Epoprostenol;
pharmacology;
Heparin;
pharmacology;
Humans;
Male;
Platelet Activation;
drug effects;
Platelet Aggregation;
drug effects;
Propyl Gallate;
pharmacology
- From:
Journal of Experimental Hematology
2004;12(5):680-683
- CountryChina
- Language:Chinese
-
Abstract:
The study was purposed to explore the suitable platelet activators to be used in slide platelet aggregation test. Experiments were as follows: (1) to detect the intensity and time in 15 healthy donors' platelet aggregation tests induced by cationic propyl gallate (c-PG) and the usual platelet activators: ADP, collagen, epinephrine, arachidonic acid and ristocentin, respectively; (2) to detect the time in platelet aggregation tests of 15 healthy donors induced by c-PG and the above usual platelet activators respectively after addition of PGI2, cAMP or EDTA; (3) to detect the time in 15 healthy donors' platelet aggregation tests induced by c-PG after addition of heparin; (4) to detect the intensity and time of platelet aggregation induced by c-PG at the platelet count of (240-15) x 10(9)/L, (5) to detect the time of platelet aggregation induced by c-PG in eight patients each of whom had taken 100 mg aspirin per day for five days. The results showed that (1) c-PG reduced the strongest intensity of platelet aggregation and the time taken was appropriate, (2) c-PG was the most effective activator to reveal the inhibitive effect on platelet by PGI2, cAMP or EDTA, (3) 0.5 - 3 U/ml heparin did not significantly change the platelet aggregation induced by c-PG, (4) 15 healthy donors' platelet aggregation induced by c-PG displayed clearly on the slide until the platelet count below 30 x 10(9)/L, (5) The platelet aggregation time induced by c-PG was significantly prolonged in eight patients who had taken aspirin. In conclusion, compared to the usual platelet activators, c-PG has remarkable potential advantages when used in slide platelet aggregation test.
