Study on the changes of IL-3 and its receptor in mice with immune-mediated aplastic anemia.
- Author:
Tao HUANG
1
;
Zhen-Qiao HUANG
;
Yong-Ming ZHOU
;
Zhi-Zhong XUE
;
Ren-Bao GAN
Author Information
1. Department of Hematology, The Yue Yang Iraditional Chinese and Weseten Medicine Integrated Hospital Affiliated to Shanghai Traditional Chinese University, Shanghai 200437, China. 13331993136@133sh.com
- Publication Type:Journal Article
- MeSH:
Anemia, Aplastic;
immunology;
pathology;
Animals;
Bone Marrow;
pathology;
Interleukin-3;
analysis;
Mice;
Mice, Inbred BALB C;
Mice, Inbred DBA;
RNA, Messenger;
analysis;
Receptors, Interleukin-3;
analysis;
genetics
- From:
Journal of Experimental Hematology
2004;12(5):684-686
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to find new idea for clinical treatment of aplastic anemia. Immune-mediated aplastic anemia mice were developed, IL-3 in the supernatant with PHA stimulating splenic cells was detected by ELISA, semi-quantiting analysis of IL-3R was performed by point hybridization. The results showed that the IL-3 level in the supernatant with PHA stimulating splenic cells of immune-mediated aplastic anemia mice was higher than controls, difference between them was significant (P <0.001), while amount of IL-3 receptor by semi-quantiting analysis was lower than control significantly. In conclusion, the IL-3 receptor expression level is important for pathogenesis and treatment strategy of aplastic anemia.
