Experimental research on tyrosine-kinase inhibitor STI571 and P21WAF gene clone to treat chronic myeloid leukemia.
- Author:
Wei WANG
1
;
Bing-Zhong SUN
;
Xin-Ping LIU
;
Qi FENG
;
Zhen-Chuan SHANG
;
Yun-Xin CAO
;
Li-Bo YAO
Author Information
1. Department of Hematology, Hospital 175 of PLA, Zhangzhou, 363000, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
genetics;
Base Sequence;
Benzamides;
Blotting, Western;
Cell Cycle;
drug effects;
genetics;
Cell Proliferation;
drug effects;
Cloning, Molecular;
Cyclin-Dependent Kinase Inhibitor p21;
genetics;
metabolism;
Humans;
Imatinib Mesylate;
K562 Cells;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
genetics;
metabolism;
pathology;
Molecular Sequence Data;
Piperazines;
pharmacology;
Protein-Tyrosine Kinases;
antagonists & inhibitors;
Pyrimidines;
pharmacology;
Transfection
- From:
Journal of Experimental Hematology
2004;12(6):737-742
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effect of a tyrosine-kinase inhibitor STI571 and P21(WAF) gene clone on the proliferation, cycle, apoptosis of leukemia cell line K562, P21(WAF) gene was obtained by RT-PCR, and its sequence was approved to be correct, then P21-pcDNA3.1 vector was constructed and transfected into K562 cell line. After selected with G418, P21-pcDNA3.1-K562 cell clone that stably expression P21(WAF) was isolated. P21(WAF) protein was identified by Western blot. The survival rate were tested by MTT. Cell cycle and apoptosis were tested by flow cytometry. The results showed that the expression of P21(WAF) protein could be detected by Western blot in P21-pcDNa3.1-K562 cells. A strong inhibition of cell proliferation was observed in P21-pcDNA3.1-K562 cells as compared with that of the control. The cells cycle were arrested in G(0)/G(1) phase. The percentage of apoptosis was declined slightly after P21-pcDNA3.1-K562 cells were combined with STI571, meanwhile its survival rate declined more slowly than that of K562 cell with STI571. In conclusion, P21(WAF) inhibits the proliferation of K562 cell, meanwhile slightly inhibits its apoptosis induced by STI571and decrease its sensitivity to STI571.
