Study on large-scale CD34+ cell enrichment using immunomagnetic isolation apparatus.
- Author:
Hong LIU
1
;
Sui CAO
;
Xiu-Bao REN
;
Peng ZHANG
;
Xiu-Mei AN
;
Xi-Shan HAO
Author Information
1. Department of Immunology, Affiliated Hospital of Oncology, Tianjing Medical University, Tianjing 300060, China. abcd-lh@eyou.com
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
immunology;
Colony-Forming Units Assay;
Flow Cytometry;
Hematopoietic Stem Cells;
cytology;
immunology;
Humans;
Immunomagnetic Separation;
instrumentation;
methods;
standards;
Neoplasms;
blood
- From:
Journal of Experimental Hematology
2004;12(6):798-802
- CountryChina
- Language:Chinese
-
Abstract:
In order to find a method suitable for purifying large amount of CD34(+) cells, from 5 cases who accepted autologous peripheral blood stem cell transplantation, CD34(+) cells were collected and enriched by using Isolex 300i (Nexell). Phenotypes were detected by flow cytometry and the biological viability were assayed by the colony-forming experiments and cell expansion experiment in vitro. The results showed that the number of mononuclear cells first collected was about (3.5 - 6.0) x 10(10) and (0.55 - 1.2)% of cells were CD34 positive. The number of positive production was about (2.0 - 3.0) x 10(8); the CD34(+) cells purity was (75 - 85)% and the yield was (40 - 65)%. The CD34(+) cells of positive production could expand up to 2 - 3 times when cultured with SCF + IL3 + FL + TPO + EPO in vitro. The results of colony-forming experiments demonstrated that the CD34(+) cells collected has enough colony-forming ability. All results showed the enriched CD34(+) cells with biological viability. In conclusion, the CD34(+) immunomagnetic isolation apparatus Isolex300i is suitable to clinical application for a large amount of CD34(+) cell enrichment.
