Establishment and evaluation on the method for platelet intracellular trehalose determination.
- Author:
Jun ZHOU
1
;
Jing-Han LIU
;
Xi-Lin OUYANG
;
Yuan ZHUANG
;
Xi-Jin LI
Author Information
1. Department of Blood Transfusion, Gerneral Hospital of PLA, Center of Clinical Transfusion of PLA, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Blood Platelets;
chemistry;
Chromatography, High Pressure Liquid;
methods;
Humans;
Reproducibility of Results;
Sulfuric Acids;
chemistry;
Trehalose;
analysis
- From:
Journal of Experimental Hematology
2004;12(6):837-840
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish an applicable, convenient and rapid method for platelet intracellular trehalose determination, so that the technique of trehalose loading could be optimized and used in research on freeze-drying platelets. Protein from loaded-trehalose platelets was deposited by using trichloroacetic acid, trechalose concentration in platelets was determinated by sulfuric-anthrone reaction and was analyzed by high performance liquid chromatography (HPLC). The results showed that when platelets were loaded with 1.7% of trehalose in loading solution, intracellular concentration determinated by sulfuric-anthrone reaction was 0.22% and derterminated by HPLC was 0.2%. The recovery rate of trehalose determinated by sulfuric-anthrone reaction was 100.7% and stability and repeatability of results were good. In conclusion, the method is convenient, rapid, accurate and highly sensitive for determination of platelet intracellar trehalose.
