Effects of HGF on GVHD and Th1/Th2-related cytokines in ALL mice after allo-BMT.
- Author:
Yun-Jin XIA
1
;
Qing-Ping GAO
;
Chu-Cheng WAN
;
Fan-Jun CHENG
;
Wei-Min WANG
;
Ren-Ci GUO
Author Information
1. Department of Hematology, Taihe Hospital of Yunyang Medical College, Shiyan 442000, China. xiayunjin@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Transplantation;
adverse effects;
methods;
Cytokines;
blood;
Enzyme-Linked Immunosorbent Assay;
Female;
Graft vs Host Disease;
immunology;
prevention & control;
Hepatocyte Growth Factor;
pharmacology;
Interferon-gamma;
blood;
Interleukin-4;
blood;
Male;
Mice;
Mice, Inbred BALB C;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
blood;
immunology;
surgery;
Th1 Cells;
immunology;
Th2 Cells;
immunology;
Transplantation, Homologous
- From:
Journal of Experimental Hematology
2005;13(1):35-38
- CountryChina
- Language:Chinese
-
Abstract:
To observe the effects of hepatocyte growth factor (HGF) on graft-versus-host disease (GVHD) and Th1/Th2 related cytokines in mice with acute lymphoblastic leukemia (ALL) after allogenic bone marrow transplantation (allo-BMT), BALB/c mice were conditioned by total body irradiation with 11 Gy and then were transplanted with allogeneic bone marrow after establishing ALL model. BALB/c mice were divided into groups A and B. The mice of group A were injected subcutaneously with HGF from day 0 to 7 after allo-BMT, and the mice of group B were injected subcutaneously with PBS from day 0 to 7 after allo-BMT. The symptoms of GVHD and the GVHD pathological changes of liver and small intestine and skin were observed. The serum levels of both IFN-gamma and IL-4 were determined by ELISA. The results showed that the score of GVHD in group A was lower than that in group B (P < 0.05). The levels of IFN-gamma in both groups A and B were all higher than that in normal group (P < 0.05 and P < 0.001, respectively), However, the level of IFN-gamma in group A was lower than that in group B (P < 0.01). The levels of IL-4 in both group A and B were all lower than that in normal group (P < 0.05), but the level of IL-4 in group A was higher than that in group B (P < 0.05). It is concluded that HGF can alleviates the severity of GVHD, because of its balancing the Th1/Th2-related cytokines after allo-BMT.
