Osteoblasts derived from mesenchymal stem cells harbor immunoregulatory effect.
- Author:
Xiao-Xia JIANG
1
;
Yi ZHANG
;
Xiu-Sen LI
;
Ying WU
;
Xiao-Dan YU
;
Pei-Hsien TANG
;
Ning MAO
Author Information
1. Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
cytology;
immunology;
Cell Differentiation;
immunology;
Cell Lineage;
Cell Proliferation;
Cells, Cultured;
Humans;
Mesenchymal Stromal Cells;
cytology;
immunology;
Osteoblasts;
cytology;
immunology;
T-Lymphocytes;
cytology;
immunology
- From:
Journal of Experimental Hematology
2005;13(1):50-53
- CountryChina
- Language:Chinese
-
Abstract:
In an attempt to study the immunoregulatory effect of osteoblasts derived from mesenchymal stem cells (MSC), MSC was induced to differentiate into osteoblasts for one week. The growth pattern and the phenotype were evaluated by MTT and flow cytometry respectively. The immunoregulatory effect was tested by the inhibitory effect on T cell proliferation. The result showed that during the differentiation cells grew fast and there was no significant change in the phenotypes but keeping CD73, CD105, CD44, CD29 positive and CD34, CD45, HLA-DR, CD86 negative. Osteocyte derived from MSC also showed immunosuppressive effect on T cell proliferation in adose-dependent manner. It is concluded that osteoblasts derived from MSC also harbored immunoregulatory effect.
