Expression and its significance of TRAIL and its receptors in cells of patients with acute myeloid leukemia.
- Author:
Shi ZHAO
1
;
Hong-Xiang WANG
;
Hong MAO
;
Juan XIAO
;
Ping ZOU
Author Information
1. Department of Hematology, Wuhan Central Hospital, Wuhan 430014, China. ljwhx@21cn.com
- Publication Type:Journal Article
- MeSH:
Adult;
Female;
Flow Cytometry;
Gene Expression Regulation, Leukemic;
Humans;
Leukemia, Myeloid, Acute;
genetics;
metabolism;
pathology;
Male;
Middle Aged;
RNA, Messenger;
biosynthesis;
genetics;
Receptors, TNF-Related Apoptosis-Inducing Ligand;
biosynthesis;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
TNF-Related Apoptosis-Inducing Ligand;
biosynthesis;
genetics
- From:
Journal of Experimental Hematology
2005;13(1):65-69
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to detect the expression of TNF related apoptosis-inducing ligand (TRAIL) and its receptors on acute myeloid leukemic (AML) cells, and explore its possible role in leukemia therapy. RT-PCR and flow cytometry were used to detect the expression of TRAIL and its receptors on AML cells of 39 cases (patient group), AML cells of 18 cases with complete remission (CR group) and BMMNC or PBMNC of 21 normal persons (control group). The results showed that (1) TRAIL, DR4 and DR5 were highly expressed in both patient group and CR group, while the DcR1 and DcR2 were poorly expressed. (2) The level of DR5 expression in CR group was higher than that in patient group. (3) The level of DR5 was higher than DR4 in both patient group and CR group. (4) TRAIL and its receptors were expressed similarly in different subtypes of AML. In conclusion, there are differences between the expressions of TRAIL and its receptors in AML cells. DR5 may play an important role in TRAIL-inducing apoptosis of AML cells.
