Whole exon 5 and intron 5 replaced by RHD/CE in partial D phenotype DVa (Hus).
- Author:
Yi-Yan ZHOU
1
;
Wen XIONG
;
Chao-Peng SHAO
Author Information
1. Shenzhen Blood Center, Shenzhen 518035, China. yyzhou@szbc.org
- Publication Type:Journal Article
- MeSH:
China;
Exons;
genetics;
Genotype;
Humans;
Introns;
genetics;
Phenotype;
Polymerase Chain Reaction;
Rh-Hr Blood-Group System;
genetics;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2005;13(1):140-142
- CountryChina
- Language:Chinese
-
Abstract:
The study was purposed to analyze DNA and allele structure of the partial D phenotypes D(Va) and D(VI) of the Rhesus blood group in Chinese. Through polymerase chain reaction (PCR) and direct genomic DNA sequencing, the RHD gene was detected in three weak D individuals identified serologically. The results showed that among the three weak D individuals, one was identified as partial D phenotype D(Va) (Hus) type and genotyped DccEe; another two were testified as D(VI) III type and genotyped DCcee. Moreover, the breakpoints of the replaced region by RHCE in D(Va) (Hus) were 5' end of the exon 5 and 3' end of the intron 5, and there were 7 novel polymorphisms in intron 5: 23-25(GCA)2, 98G>A, 168-169insG, 205-206insT, 494-495insA, 1256-1257insC, 1347G>T. In conclusion the whole exon 5 and intron 5 are replaced by RHCE in D(Va) (Hus) detected in Chinese.
