Quantification of human ermap by using real-time FQ-PCR.
- Author:
Xiao-Hong ZHANG
1
;
Tie-Zhen YE
;
Bin HU
;
Wen-Zhang SI
Author Information
1. Department of Pediatrics, Conghua Central Hospital, Guangzhou 510089, China.
- Publication Type:Journal Article
- MeSH:
Blood Group Antigens;
genetics;
Butyrophilins;
DNA, Complementary;
chemistry;
genetics;
Fluorescent Dyes;
chemistry;
Fluorometry;
methods;
Humans;
Polymerase Chain Reaction;
methods;
Reproducibility of Results
- From:
Journal of Experimental Hematology
2005;13(1):154-157
- CountryChina
- Language:Chinese
-
Abstract:
To develop a real-time FQ-PCR method for quantifying human ermap, a set of primers and a fluorescent probe were designed by primer express 2.0. pBluescriptSK(+) plasmid contained ermap cDNA was transcribed to generate calibration standards for quantification. A real time FQ-PCR method was established. The results showed that when the concentrations of DNA to be amplified were ranged from 1.725 x 10(7) to 1.725 x 10(10) cps/ml, there was a good correlation between template concentration and cycle threshold, and the correlation coefficient reached to -0.999376. In conclusion, real time FQ-PCR which is specific, sensitive and accurate can be used to further research on human ermap.
