Detection of chromosomal aberration in paraffin-embedded synovial sarcoma tissues by fluorescence in-situ hybridization.
- Author:
Yu-fan CHENG
1
;
Jian WANG
;
Xiao-yan ZHOU
;
Xue-ke ZHOU
;
Yong-ming LU
;
Da-ren SHI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Biomarkers, Tumor; genetics; Child; Child, Preschool; Chromosome Aberrations; Female; Head and Neck Neoplasms; genetics; metabolism; Humans; In Situ Hybridization, Fluorescence; Lower Extremity; pathology; Male; Middle Aged; Oncogene Proteins, Fusion; genetics; Paraffin Embedding; Reverse Transcriptase Polymerase Chain Reaction; Sarcoma, Synovial; genetics; metabolism; Soft Tissue Neoplasms; genetics; metabolism; Young Adult
- From: Chinese Journal of Pathology 2007;36(9):577-581
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the application of fluorescence in-situ hybridization (FISH) in detection of gene translocation in paraffin-embedded tissue samples of synovial sarcoma.
METHODSInterphase FISH was carried out in paraffin-embedded tissue of 42 cases of synovial sarcoma and 9 cases of non-synovial sarcoma, using a LSI SYT (18q11.2) dual color break-apart probe. In all of the cases studied, the gene fusion product SYT-SSX was also analyzed by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSPositive signals were detected in 37 cases (88.1%) of synovial sarcoma by FISH, as compared with 35 cases (83.8%) by RT-PCR and 39 cases (92.9%) by both techniques. Of the 39 positive cases, 33 cases (78.5%) revealed SYT gene translocation.
CONCLUSIONSFISH may serve as an adjunctive diagnostic tool in problematic cases of synovial sarcoma and can be applied in paraffin-embedded tissue samples. As compared with RT-PCR, FISH is also sensitive and reliable. The methodology is less labor intensive and time consuming. FISH has great potential in molecular diagnosis of soft tissue tumors.
