Expression of matrix metalloproteinase-2, -9 and their inhibitor-1 in hypertrophic scars.
- Author:
Xiao-Fan XIE
1
;
Li-Xin HE
;
Xiao-Feng HAO
;
Bi CHEN
;
Chi-Yu JIA
;
Zhi-Gang SUN
;
Yu-Jue CAO
;
Dong Hai LI
Author Information
- Publication Type:Journal Article
- MeSH: Cicatrix, Hypertrophic; genetics; metabolism; pathology; Female; Gene Expression; Humans; Male; Matrix Metalloproteinase 2; genetics; metabolism; Matrix Metalloproteinase 9; genetics; metabolism; RNA, Messenger; metabolism; Skin; metabolism; pathology; Tissue Inhibitor of Metalloproteinase-1; genetics; metabolism
- From: Chinese Journal of Burns 2007;23(6):444-446
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the gene expression of matrix metalloproteinases (MMP-2, MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in proliferative and mature hypertrophic scars.
METHODSTotal RNA from 8 normal skin samples and from 16 human hypertrophic scar samples of different maturing stage was respectively extracted, and then mRNA was isolated. The gene expressions of MMP-2, MMP-9 and TIMP-1 in these samples were examined with reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSThe gray scale ratio of MMP-2, MMP-9 and TIMP-1 transcription in normal skin were (3.8 +/- 0.7)%, (5.8 +/-4.4)%, (30.3 +/- 3.0)%, respectively, which were obviously higher than those in proliferative hypertrophic scar [(14 +/- 5)%, (18 +/- 5)%, (38 +/- 4)%, P < 0.05]. The expression of MMP-2 and MMP-9 genes in mature hypotrophic scar returned to normal level, but that of TIMP-1 remained high when compared with that of normal level (P < 0. 05).
CONCLUSIONThe increase in MMP-2, MMP-9 and TIMP-1 gene expression might be involved in the formation of hypertrophic scars, while the lowering of MMP-2 and MMP-9 gene expression might be associated with the maturation of hypertrophic scars.
