Study on Acinetobacter baumannii plasmid with 3 types of beta-lactamase genes in a burn ward.
- Author:
Rong LI
1
;
Wen-Lin LI
;
Xiao-Yu SHI
;
Yuan-Lin ZENG
;
Xiao-Wen XV
;
Lin ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Acinetobacter Infections; Acinetobacter baumannii; enzymology; genetics; isolation & purification; Burns; microbiology; Drug Resistance, Multiple, Bacterial; genetics; Genes, Bacterial; Humans; Microbial Sensitivity Tests; Plasmids; beta-Lactamases; genetics
- From: Chinese Journal of Burns 2008;24(2):97-98
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the transferrable character of Acinetobacter baumannii (AB) plasmids with 3 types of beta-lactamase gene.
METHODSThe plasmid of multi-drug resistant AB (donor) isolated from burn wound were transferred to E. coil ATCC25922 (receptor) through conjugation, and drug sensitivity was also observed. Drug-resistant gene and stability of filial generation and zygote were analyzed by PCR.
RESULTSThe drug-resistance of donor plasmids to Sulfamethoxazole, Ampicillin, Cefalotin, Cefpodoxime, Cefuroxime, Imipenem/Cilastatin and Ampicillin/Sulbactam, and three types of beta-lactamase gene were transferred to the receptor, and were also stably transmitted for passages. The minimum inhibitor concentration of receptor to Sulfamethoxazole was > 2 mg/L after conjugation with donor, and inhibitory character could be transferred to next generation.
CONCLUSIONbla(TEM-1), bla(PER-1) and bla(OXA-23) genes carried in the plasmid of AB can be transferred through conjugation and stably transmitted for passages, and it is one of the molecular mechanisms for AB with multi-drug resistance after burn infections.
