Influence of Wnt-1 recombinant adenovirus on differentiation of human epidermal stem cells.
- Author:
Yuan-chao LI
1
;
Jin-jin WU
;
Jian-jun BI
;
Tao YANG
;
Gui-hong YANG
;
Bing-qiang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Cell Differentiation; Cell Line; Epithelial Cells; cytology; virology; Humans; Matrix Metalloproteinase 2; metabolism; Matrix Metalloproteinase 7; metabolism; Stem Cells; cytology; Wnt1 Protein; genetics
- From: Chinese Journal of Burns 2008;24(3):187-190
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the influence of Wnt-1 recombinant adenovirus on differentiation tendency of human epidermal stem cells.
METHODSWnt-1 recombinant adenovirus was transduced into hESCs (E group), while normal hESCs were used as control (C) group. The diameter, proliferation,and labeling molecular expression of hESC were determined. The content of MMP-2 and MMP-7 in supernate were also assayed.
RESULTSThere was no obvious difference in diameter of hESC between two groups. The density of hESC in E group was (1.45 +/- 0.09) x 10(5)/mL, which was obviously higher than that in C group [(1.18 +/- 0.10) x 10(5)/mL, P < 0.05]. There were no obvious differences in expression of markers between two groups,including keratin 5 (KS), K6, K7, KS, K14, CD44, carcinoembryonic-like antigen (CEAA), ER, PR (P > 0.05) ,while the expression of K 10 was different among groups [(60 +/- 3)% in E group, 0 in C group], also K18 [(34.3 +/- 2.1)% in E group vs. (13.8 +/- 1.7)% in C group, P < 0.05], and K19 [(17.1 +/- 1.8)% in E group vs. (24.4 +/- 1.5)% in C group, P < 0.05].The contents of MMP-2 and MMP-7 in E group were higher than those in C group (P < 0.01).
CONCLUSIONWnt-1 recombinant adenovirus can induce the differentiation of hESCs to glandular epithelium-like cells.
