Protective effect of lytic cocktail (inhibition of stress) on lung injury in severe burn rat.
- Author:
Liang QIAO
1
;
Ke-jian YUAN
;
Hui-zhong YANG
;
He-liang DONG
;
Wen-kui WANG
;
Wei-shi XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Burns; complications; therapy; Fluid Therapy; Interferon-gamma; metabolism; Lung Injury; etiology; metabolism; therapy; Malondialdehyde; metabolism; Meperidine; therapeutic use; Peroxidase; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Tumor Necrosis Factor-alpha; metabolism
- From: Chinese Journal of Burns 2008;24(4):251-253
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of inhibition of stress (lytic cocktail) on lung injury in severe burn rats at early stage.
METHODSSprague-Dawley rats inflicted with 30% TBSA full-thickness burn were randomly divided into A group (n = 36, fluid resuscitation with administration of lytic cocktail), B group (n = 36, fluid resuscitation only). Lung function was evaluated by partial pressure of oxygen (PaO2) in arterial blood and histopathologic changes on 3, 5, 7, 10 post burn day (PBD). The levels of malonyldialdehyde (MDA), myeloperoxidase (MPO), tumor necrosis factor-alpha(TNF-alpha) and interferon-gamma (IFN-gamma) in lung tissue were measured at the same time points.
RESULTSThe PaO2 level in A group on 3 PBD (12.58 +/- 0.41 kPa) was significantly higher than that in B group (8.86 +/- 0.23 kPa, P < 0.01). Compared with those in B group, the levels of MDA and MPO were significantly decreased in A group at each time point (P < 0.05 or 0.01), the levels of TNF-alpha on 3, 5, 7 PBD (P < 0.05 or 0.01) and IFN-gamma on 5, 7, 14 PBD (P < 0.01) were also decreased in A group. Swollen lung mesenchyme was alleviated, infiltration of inflammatory cell was lessened in A group.
CONCLUSIONLytic cocktail combined with immediate fluid resuscitation can inhibit stess response, downregulate the expression of inflammatory factor, ameliorate lung function in severe burn rat at early stage.
