Expression of IL-8, SP-A and TGF-beta1 in bronchoalveolar lavage fluid of neonates with bronchopulmonary dysplasia.
- Author:
Dong-Yun LIU
1
;
Jing WU
;
Xiao-Ying ZHANG
;
Zhi-Chun FENG
Author Information
- Publication Type:Journal Article
- MeSH: Bronchoalveolar Lavage Fluid; chemistry; Bronchopulmonary Dysplasia; metabolism; Enzyme-Linked Immunosorbent Assay; Female; Humans; Infant, Newborn; Interleukin-8; analysis; physiology; Male; Pulmonary Surfactant-Associated Protein A; analysis; physiology; Transforming Growth Factor beta1; analysis; physiology
- From: Chinese Journal of Contemporary Pediatrics 2010;12(6):444-446
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of interleukin 8 (IL-8), surfactant protein-A (SP-A) and transforming growth factor beta1 (TGF-beta1) in bronchoalveolar lavage fluid (BALF) of neonates with bronchopulmonary dysplasia (BPD).
METHODSThirty neonates with BPD and 30 gestational age-, gender-, and birth weight-matched neonates without BPD (control group) were enrolled from December 2007 to October 2009. Non-brochoscopic bronchoalveolar lavage was performed. The levels of IL-8, SP-A and TGF-beta1 in BALF were measured using ELISA.
RESULTSThe levels of TGF beta1 (47+/-15 microg/mL vs 34+/-13 microg/mL) and IL-8 (54+/-16 microg/mL vs 28+/-13 microg/mL) in the BPD group were significantly higher than those in the control group (P<0.01). In contrast, the contents of SP-A in the BPD group were significantly lower than those in the control group (35+/-16 microg/mL vs 42+/-14 microg/mL;P<0.05).
CONCLUSIONSThe increased expression of TGF-beta1 and IL-8 in BALF may be involved in abnormal lung development and maturation in neonates with BPD. The low expression of SP-A in the BPD group suggests that the exogenous SP-A administration may be an option for the treatment of BPD.
