Determination and clinical significance of tissue inhibitors of metalloproteinase-1 and -2 in serum of children with nonalcoholic fatty liver disease.
- Author:
Yu-Wen LI
1
;
Chuan-Long ZHU
;
Ren-Tao GAO
;
Jia-Hua PAN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Alanine Transaminase; blood; Child; Enzyme-Linked Immunosorbent Assay; Fatty Liver; blood; Female; Humans; Male; Tissue Inhibitor of Metalloproteinase-1; blood; Tissue Inhibitor of Metalloproteinase-2; blood; gamma-Glutamyltransferase; blood
- From: Chinese Journal of Contemporary Pediatrics 2010;12(6):455-458
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine serum tissue inhibitors of metalloproteinases (TIMP) -1 and -2 levels in children with nonalcoholic fatty liver disease and to investigate possible roles of the two markers.
METHODSOne hundred and five obese children were classified into 4 groups: simple obesity (n=44), simple nonalcoholic fatty liver (SNAFL, n=25), and nonalcoholic steatohepatitis (NASH, n=36). Serum TIMP-1 and -2 levels were measured using ELISA. Serum ALT and gamma-GT levels were measured with totally automatic enzymatic method.
RESULTSSerum levels of TIMP-1 and gamma-GT increased with the disease development from simple obesity to SNAFL and NASH (P<0.05). Both serum TIMP-1 and -2 levels were positively correlated with gamma-GT levels (r=0.534, P<0.01; r=0.351, P<0.05, respectively). Ninety-seven percent of children in the NASH group had serum TIMP-1 levels over 2 standard deviations of healthy controls (83.35 microg/ L) compared with 76% in the SNAFL group (P<0.05). There were no significant differences in the case proportion with TIMP-2 levels over 2 standard deviations of healthy controls between the NASH and the SNAFL groups.
CONCLUSIONSBoth TIMP-1 and -2 may reflect the state of liver fibrosis in children with nonalcoholic fatty liver disease, and serum TIMP-1 appears to be more reliable.
