Effects of Ucf-101 on expression of Omi/HtrA2 in kidneys of postasphyxial neonatal rats.
- Author:
Bing HUA
1
;
Wen-Bin DONG
;
Qing-Ping LI
;
Zhi-Qiang FENG
;
Hong YU
;
Xue-Song ZHAI
;
Xiao-Ping LEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Apoptosis; drug effects; Asphyxia Neonatorum; drug therapy; metabolism; pathology; Female; High-Temperature Requirement A Serine Peptidase 2; Humans; Immunohistochemistry; In Situ Nick-End Labeling; Infant, Newborn; Kidney; chemistry; Male; Mitochondrial Proteins; analysis; antagonists & inhibitors; Pyrimidinones; pharmacology; therapeutic use; Rats; Rats, Wistar; Serine Endopeptidases; analysis; Thiones; pharmacology; therapeutic use
- From: Chinese Journal of Contemporary Pediatrics 2010;12(8):658-661
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of serine protease Omi/HtrA2 in kidneys of postasphyxial neonatal rats, and to study the effects of Ucf-101 on apoptosis and the expression of Omi/HtrA2 in these rats.
METHODSSeventy-two neonatal Wistar rats of 7-10 days old were randomly divided into 3 groups: control, postasphyxial model, Ucf-101-treated postasphyxialThe postasphyxial model was established by normobaric asphyxiaExpression of Omi/HtrA2 was determined with streptavidin-peroxidase immunohistochemistry 2, 24 and 48 hrs after asphyxia. Terminal deoxynuleotidyl-mediated nick end labeling (TUNEL) was used to ascertain the apoptosis of renal cells.
RESULTSCompared with the control group, OmiHtrA2 expression in renal cells began to increase 2 hrs after asphyxia and peaked at 24 hrs. The expression of Omi/HtrA2 in the Ucf-101-treated postasphyxial group was significantly lower than that in the postasphyxial model group (P<0.01). TUNEL-positive cells began to increase 2 hrs after asphyxia and peaked at 24 hrs in the postasphyxial model group when compared with the control group. The number of TUNEL-positive cells in the Ucf-101-treated postasphyxial group was significantly lower than that in the postasphyxial model group at all time points (P<0.01).
CONCLUSIONSThe expression of Omi/HtrA2 in kidneys is increased in postasphyxial neonatal rats. The increased Omi/HtrA2 expression may play an important role in the development of postasphyxial renal injury. Treatment with Ucf-101 can reduce the expression of Omi/HtrA2 in kidneys of postasphyxial neonatal rats and thus reduce renal tububar epithelial cell apoptosis.
