Bio-panning of antagonistic peptides against HAb18G/CD147 and their function of anti-hepatoma invasion.
- Author:
Bao-cheng HUANG
1
;
Peng SHANG
;
Ai-rong QIAN
;
Xian-hui WANG
;
Guang-hua SHI
;
Zhi-nan CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; therapeutic use; Basigin; metabolism; Carcinoma, Hepatocellular; drug therapy; pathology; Humans; Liver Neoplasms; drug therapy; pathology; Mice; Neoplasm Invasiveness; Peptide Library; Peptides; therapeutic use
- From: Chinese Journal of Oncology 2003;25(2):111-114
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen out the HAb18G/CD147 binding peptides and find out an antagonist against hepatoma invasion.
METHODSHAb18G/CD147 was purified by affinity chromatographic method and the antigen binding peptides acquired by bio-panning a phage-displayed 12-peptide library. After obtaining the sequence of the selected phage-displayed peptides, all the 9 peptides were synthesized by solid-phase method and identified by mass spectrograph. The peptides' anti-metastatic function was tested by Boyden Chamber assay.
RESULTSThe purified HAb18G/CD147, identified by Western blot (molecular weight about 65 kd) could be used to bio-pan the phage-displayed peptide library. After 3 rounds of bio-panning, 9 positive phage clones were selected and sequenced. The synthesized peptides had uneven inhibitory activities and three of them were able to markedly inhibit the hepatoma cell invasion (P < 0.01). The most effective peptide decreased by 90.1% of hepatoma cells migrating through the Boyden Chamber membrane as compared with the control.
CONCLUSIONBio-panning the phage-displayed peptide library can be used successfully to screen out the antigen binding peptides. Hepatoma metastatic potential can be inhibited by peptide antagonist which could be a good foundation of developing peptide therapeutic agent against hepatoma metastasis.
