Establishment of human multidrug-resistant lung carcinoma cell line (D6/MVP).
- Author:
Sheng-lin MA
1
;
Jian-guo FENG
;
Lin-hui GU
;
Yu-tian LING
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; analysis; Antineoplastic Combined Chemotherapy Protocols; administration & dosage; therapeutic use; Cell Line, Tumor; Cisplatin; administration & dosage; Drug Resistance, Multiple; Flow Cytometry; Glutathione; analysis; Glutathione Transferase; metabolism; Humans; Lung Neoplasms; drug therapy; pathology; Mitomycins; administration & dosage; Vinblastine; administration & dosage
- From: Chinese Journal of Oncology 2003;25(2):134-136
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish human multidrug-resistant lung carcinoma cell line (D6/MVP) with its characteristics studied.
METHODSIntermittent administration of high-dose MMC, VDS and DDP (MVP) was used to induce human lung carcinoma cell line (D6) to a multidrug-resistant variety (D6/MVP). MTT assay was used to study the multidrug resistance of D6/MVP to multianticarcinogen. Flow cytometry was used to study the cell cycle distribution and the expression of P-gp, multidrug resistance-associated protein (MRP) and GSH/GST.
RESULTS1. D6/MVP was resistant to many anti-tumor agents, with the IC(50) 13.3 times higher and the drug resistance 2 - 6 times higher than D6, 2. The multiplication time of D6/MVP was prolonged and the cell number of S-phase decreased while that of G1- and G(2)-phase increased and 3. The expression of P-gp and MRP was enhanced significantly (96.2% vs 51.7%), but the expression of GSH/GST kept stable.
CONCLUSIOND6/MVP is a multidrug-resistant cell line possessing the basic characteristics of drug-resistance.
