Tissue microarray and in situ hybridization study on hypoxia inducible factor 1alpha mRNA expression in epithelial ovarian tumor.
- Author:
Jun LUO
1
;
Zhi-lan PENG
;
He WANG
;
Yi-fan CHENG
;
Kai-xuan YANG
;
Lan XIE
;
Xiao-lei QIAN
;
Yan ZOU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Female; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; analysis; genetics; In Situ Hybridization; methods; Middle Aged; Neoplasms, Glandular and Epithelial; chemistry; metabolism; Ovarian Neoplasms; chemistry; metabolism; Ovary; metabolism; RNA, Messenger; analysis; Tissue Array Analysis; methods
- From: Chinese Journal of Oncology 2003;25(2):168-170
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression and significance of hypoxia inducible factor 1alpha (HIF-1alpha) in epithelial ovarian tumors.
METHODSThe expression of HIF-1alpha mRNA in 295 patients with epithelial ovarian tumor was analyzed retrospectively by high-throughput tissue microarray and in situ hybridization, which was compared with 13 normal ovarian tissue samples.
RESULTSThe expression rates of HIF-1alpha mRNA were 0, 13.2%, 42.1% and 81.9% in normal ovarian tissue, benign, borderline and malignant ovarian tumors. Expression rate of HIF-1alpha mRNA in borderline and invasive tumor was significantly higher than those in normal ovarian tissue and benign tumor (P < 0.001). Statistical analysis revealed that the expression of HIF-1alpha mRNA was not related to FIGO stages or histological subtypes. Close negative relation was observed between the expression of HIF-1alpha mRNA and tumor histological differentiation (P < 0.001).
CONCLUSIONThe overexpression of HIF-1alpha may play an important role in oncogenesis of epithelial ovarian tumor. Tissue microarray is an efficient technique of molecular biology.
