Antisense oligodeoxynucleotides of human telomerase reverse transcriptase inhibit endometrial carcinoma cell HEC-1A proliferation.
- Author:
Xue-jun CHEN
1
;
Wei ZHENG
;
Sheng-qi WANG
Author Information
- Publication Type:Journal Article
- MeSH: Caspases; metabolism; Cell Line, Tumor; Cell Proliferation; Endometrial Neoplasms; pathology; therapy; Female; Genetic Therapy; Humans; Oligonucleotides, Antisense; genetics; RNA, Messenger; analysis; Telomerase; antagonists & inhibitors; genetics
- From: Chinese Journal of Oncology 2003;25(3):212-215
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate antisense technology for human telomerase inhibition in the treatment of endometrial cancer.
METHODSAn antisense oligodeoxynucleotides (AODN) directed against the human telomerase transcriptase (hTERT), designed and synthesized to serve as a telomerase inhibitor, was transfected into endometrial carcinoma cell line HEC-1A by lipofectin. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to test the expression of hTERT mRNA and hTERT protein before and after transfection. Telomerase activity was tested by telomeric repeat amplification protocol. The proliferation and growth of HEC-1A were also studied by methyl thiazolyl tetrazolium and cell growth curve before and after transfection.
RESULTSAODN could down-regulate the expression of hTERT mRNA and protein, inhibiting telomerase activity and proliferation of endometrial cancer cell line in a dose- and period-dependent manner.
CONCLUSIONAntisense oligodeoxynucleotides of human telomerase transcriptase definitely inhibits the proliferation of endometrial cancer cell line. Telomerase inhibitor may thus become a new gene therapeutic agent for endometrial carcinoma.
